Journal article
Radloimmunoassay of intestinal goblet cell mucin
Abstract
A double-antibody radioimmunoassay was developed for the measurement of rat intestinal goblet cell mucin. Labeling of the mucin was achieved by mild periodate oxidation and sodium boro[3H]hydride reduction using the technique of Van Lenten and Ashwell [(1971)] J. Biol. Chem.246, 1889–1894]. Tracer amounts (0.5 ng) of labeled mucin were incubated with specifie rabbit antibody in the presence of unlabeled mucin standards or extracts of intestinal …
Authors
Forstner JF; Ofosu F; Forstner GG
Journal
Analytical Biochemistry, Vol. 83, No. 2, pp. 657–665
Publisher
Elsevier
Publication Date
12 1977
DOI
10.1016/0003-2697(77)90070-7
ISSN
0003-2697