Journal article
A Barbourin-albumin Fusion Protein that Is Slowly Cleared In Vivo Retains the Ability to Inhibit Platelet Aggregation In Vitro
Abstract
Barbourin is a 73 amino acid venom protein that inhibits platelet aggregation. Recombinant barbourin (BARH6), rabbit serum albumin (RSAH6), and a barbourin-RSA fusion protein (barbourin-linker-albumin; BLAH6) were secreted from Pichia pastoris yeast, and purified by nickel-chelate affinity chromatography via their C-terminal hexahistidine (H6) tags. BARH6 and BLAH6 did not differ in their IC50s for inhibition of platelet aggregation using …
Authors
Marques JA; George JK; Smith IJ; Bhakta V; Sheffield WP
Journal
Thrombosis and Haemostasis, Vol. 86, No. 03, pp. 902–908
Publisher
Thieme
Publication Date
2001
DOI
10.1055/s-0037-1616148
ISSN
0340-6245
Associated Experts
Fields of Research (FoR)
Medical Subject Headings (MeSH)
Adenosine DiphosphateAnimalsBase SequenceCloning, MolecularCrotalid VenomsDNA, ComplementaryHalf-LifeHumansMetabolic Clearance RateMolecular Sequence DataPichiaPlatelet AggregationPlatelet Aggregation InhibitorsPlatelet Glycoprotein GPIIb-IIIa ComplexRabbitsRecombinant Fusion ProteinsSerum AlbuminThrombin