Journal article
Simple Fluorescent Sensors Engineered with Catalytic DNA ‘MgZ’ Based on a Non-Classic Allosteric Design
Abstract
Most NAE (nucleic acid enzyme) sensors are composed of an RNA-cleaving catalytic motif and an aptameric receptor. They operate by activating or repressing the catalytic activity of a relevant NAE through the conformational change in the aptamer upon target binding. To transduce a molecular recognition event to a fluorescence signal, a fluorophore-quencher pair is attached to opposite ends of the RNA substrate such that when the NAE cleaves the …
Authors
Chiuman W; Li Y
Journal
PLOS ONE, Vol. 2, No. 11,
Publisher
Public Library of Science (PLoS)
DOI
10.1371/journal.pone.0001224
ISSN
1932-6203