Expression of the sodium‐glucose cotransporter SGLT1 gene along the jejunal crypt‐villus axis measured by quantitative real time RT‐PCR in the formula‐fed neonatal pig

This study was conducted to quantify the SGLT1 mRNA expression along the jejunal crypt‐villus axis in fed neonatal pigs. Six Yorkshire gilts were removed from sows at d 5 of age and fed a milk‐based liquid formula in until 14–16 d of age before being sacrificed for tissue collection. Three epithelial cell fractions, representing cells from the upper villus, the middle villus and the crypt regions, were sequentially isolated, with cell viability …