Novel tangential-flow countercurrent cascade ultrafiltration configuration for continuous purification of humanized monoclonal antibody

This paper discusses the use of a novel tangential-flow, three-stage, countercurrent cascade ultrafiltration configuration for continuous purification of monoclonal antibody Campath-1H from simulated mammalian cell culture supernatant. The main limitation of conventional ultrafiltration based bioseparation, particularly when using a single-stage system is the trade-off between recovery and purity. The aim of this study was to try to improve …