Fractionation of monoclonal antibody aggregates using membrane chromatography

Monoclonal antibodies (mAbs) comprise an important class of biopharmaceuticals. Aggregation of mAbs is a common though undesirable occurrence. Size exclusion chromatography (SEC) which is used for both analysis and preparative separation of mAb aggregates is slow and results in poorly resolved peaks, particularly for higher order aggregates. We describe a hydrophobic interaction membrane chromatography (HIMC) based method for rapid and …