Using Sugar and Amino Acid Additives to Stabilize Enzymes within Sol−Gel Derived Silica

The inclusion of additives during the immobilization of proteins into sol−gel processed materials has been widely explored as a route to stabilize proteins against the denaturing stresses encountered upon entrapment. In this report, we explore the effects of sorbitol and N-methylglycine (collectively referred to as osmolytes) on both the conformational stability and biological activity of the enzymes α-chymotrypsin and ribonuclease T1 in …