Optimization of Sol−Gel Formulations and Surface Treatments for the Development of Pin-Printed Protein Microarrays

We report on the development and optimization of a sol−gel-based method for the preparation of protein microarrays that has the potential to allow pin-spotting of active proteins for high throughput multianalyte biosensing and screening of protein−small molecule interactions. Microarrays were printed onto bare and chemically modified surfaces using the commercially available sol−gel precursors tetraethyl orthosilicate and sodium silicate and …