Probing the Origins of Spectroscopic Responses to Analyte-Induced Conformational Changes in Fluorescently-Labeled Cod III Parvalbumin

An emerging strategy for the development of reagentless biosensors is the coupling of fluorescence responses to analyte-induced conformational changes within fluorescently-labeled proteins. In this work, we have examined the absorbance and the steady-state and time-resolved fluorescence responses to Ca2+-induced conformational changes within cod III parvalbumin (C3P), which was labeled at cysteine-18 with the fluorescent probes fluorescein, …