Measurement of intrinsic fluorescence to probe the conformational flexibility and thermodynamic stability of a single tryptophan protein entrapped in a sol–gel derived glass matrix

In this study techniques based on measurement of intrinsic tryptophan (Trp) fluorescence were used to monitor the stability and conformational flexibility of the single tryptophan protein monellin which was entrapped in sol–gel derived glass monoliths formed from tetraethylorthosilicate. The monoliths were aged either in buffer (wet-aged) or in air (dry-aged). The steady-state fluorescence spectra, steady-state anisotropy and acrylamide …