Optimization of the Self-Quenching Response of Nitrobenzoxadiazole Dipalmitoylphosphatidylethanolamine in Phospholipid Membranes for Biosensor Development

Incorporation of the lipid-conjugated fluorescent probe nitrobenzoxadiazole dipalmitoylphosphatidylethanolamine (NBD-PE) into bilayer lipid membranes (BLMs) provides a matrix wherein changes in the structure of the membrane can be transduced into changes in fluorescence intensity or lifetime. In the work reported here, a comparison was made between an empirical model recently developed by our group to account for alterations in the fluorescence …