Preparation and Entrapment of Fluorescently Labeled Proteins for the Development of Reagentless Optical Biosensors

A new class of reagentless optical biosensors are emerging based on the use of engineered proteins that are site-selectively labelled with a fluorescent reporter group. Such sensors can operate either by direct interaction of the analyte with the bound probe, or by generating a fluorescence response to an analyte induced conformational change in the protein. In this review, the preparation, characterization and use of fluorescently labelled …