Journal article
A high-resolution melting approach for analyzing allelic expression dynamics.
Abstract
Single nucleotide polymorphisms (SNPs) are single base pair mutations that provide new approaches to studies of allele transcript abundances. High-resolution DNA melting curve (HRM) analysis using a LightScanner (Hi-Res Melting system with Idaho's LC Green) provides post-PCR detection of mutations and SNPs in genomic DNA. This study investigated whether the HRM analysis can distinguish alleles among potato (Solanum tuberosum) transcript …
Authors
Yuan J; Haroon M; Lightfoot D; Pelletier Y; Liu Q; Li X-Q
Journal
Current Issues in Molecular Biology, Vol. 11 Suppl 1, , pp. i1–i9
Publisher
MDPI
Publication Date
2009
DOI
10.21775/cimb.011.0i1
ISSN
1467-3037