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Comparison of in-house and commercial 16S rRNA...
Journal article

Comparison of in-house and commercial 16S rRNA sequencing with high-performance liquid chromatography and genotype AS and CM for identification of nontuberculous mycobacteria

Abstract

Sequencing of the 16S gene or other targets and line probe assay are in wide use for the identification of nontuberculous mycobacteria. We compared in-house and commercial sequencing with 3 sequence databases against high-performance liquid chromatography (HPLC) and line probe assay (HAIN Genotype AS and CM) for the identification of 84 reference, clinical, and unique strains representing 41 species. Consensus of methods was used as reference …

Authors

Daley P; Petrich A; May K; Luinstra K; Rutherford C; Chedore P; Jamieson F; Smieja M

Journal

Diagnostic Microbiology and Infectious Disease, Vol. 61, No. 3, pp. 284–293

Publisher

Elsevier

Publication Date

7 2008

DOI

10.1016/j.diagmicrobio.2008.02.018

ISSN

0732-8893