Journal article
Optimization of a Fluorescence-based Lysozyme Activity Assay for Contact Lens Studies
Abstract
PURPOSE: To optimize a fluorescence-based lysozyme activity assay to investigate the conformational state of lysozyme in solution and to determine the impact of extraction and evaporation procedures and the possible interference of contact lens materials on lysozyme activity.
METHODS: The fluorescence-based lysozyme activity assay, Enzchek (Molecular Probes Inc, Eugene, OR) which utilizes fluorescently quenched Micrococcus lysodeikticus, was …
Authors
Ng A; Heynen M; Luensmann D; Subbaraman LN; Jones L
Journal
Current Eye Research, Vol. 38, No. 2, pp. 252–259
Publisher
Taylor & Francis
Publication Date
2 2013
DOI
10.3109/02713683.2012.757324
ISSN
0271-3683