Strategies for improving electrophoresis data from the Coulter DELSA

Different approaches to the determination of electrophoretic mobilities of colloids with the Coulter DELSA electrophoresis instrument are compared. The best procedure was to collect data near both walls of the cell and in the center of the sample chamber. The data are fitted to the Komagata equation for electro-osmotic flow to give the mobility and the electro-osmotic velocity near the cell wall. The micrometer reading corresponding to the cell …