3-d chemical imaging with STXM tomography

Three dimensional (3-d) chemical mapping using angle scan tomography in a soft X-ray scanning transmission X-ray microscope (STXM) has been applied to quantitative chemical mapping in three dimensions of calcium carbonate biomineralization by planktonic freshwater cyanobacteria of the strain Synechococcus leopoliensis PCC 7942. Aspects of making and mounting grid sections, acquiring multi-energy, multi-angle sequences, and data analysis are discussed.