Technical note: improved DNA extraction from ancient bones using silica-based spin columns. Academic Article uri icon

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abstract

  • We describe a simple method for extracting polymerase chain reaction-amplifiable DNA from ancient bones without the use of organic solvents. Bone powders are digested with proteinase K, and the DNA is purified directly using silica-based spin columns (QIAquick3, QIAGEN). The efficiency of this protocol is demonstrated using human bone samples ranging in age from 15 to 5,000 years old.

authors

  • Yang, DY
  • Eng, B
  • Waye, John
  • Dudar, JC
  • Saunders, SR

publication date

  • April 1998