Journal article
Extracellular Expression, Purification, and Characterization of a Winter Flounder Antifreeze Polypeptide from Escherichia coli
Abstract
HPLC6 is the major component of liver-type antifreeze polypeptides (AFPs) from the winter flounder, Pleuronectes americanus. To facilitate mutagenesis studies of this protein, a gene encoding the 37-amino acid mature polypeptide was chemically synthesized and cloned into the Tac cassette immediately after the bacterial ompA leader sequence for direct excretion of the AFP into the culture medium. Escherichia coli transformant with the construct …
Authors
Tong L; Lin Q; Wong WKR; Ali A; Lim D; Sung L; Hew CL; Yang DSC
Journal
Protein Expression and Purification, Vol. 18, No. 2, pp. 175–181
Publisher
Elsevier
Publication Date
March 2000
DOI
10.1006/prep.1999.1176
ISSN
1046-5928