Isolation and transplantation of oligodendrocyte precursor cells

Newborn DBA/1J mouse neopallium was disaggregated and grown in high cell densities in tissue culture. In culture, the oligodendrocyte cell precursors are recognized as small refractile cells which use astrocyte precursor cells as a substratum. Using metrizamide density gradients, the oligodendrocyte precursor cells were separated from the astroblasts after 7 days in culture and then transplanted into the cerebellums of neonatal mice. The differentiation of the cultured oligodendrocyte precursors was analyzed in the transplants by nuclear morphometry, light and electron microscopy and immunocytochemistry. Analysis of the experiments indicated that the oligodendrocyte precursor cells, initially grown in culture, differentiated and myelinated host neuronal processes after transplantation. Moreover, the ultrastructure of the transplanted oligodendrocytes resembled mature oligodendrocytes in situ.