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Optimization of the determination of...
Journal article

Optimization of the determination of 4-aminopyridine in human serum and urine by column liquid chromatography

Abstract

Two convenient reversed-phase column liquid chromatographic procedures are described for the determination of 4-aminopyridine in human serum and urine. A 0.5-ml aliquot of serum after the addition of a 0.5-ml solution of 4-(aminomethyl)pyridine in 0.1 M Na2HPO4 as the internal standard is passed through a 1-ml BondElut C18 silica extraction column. The column is selectively washed to remove acidic, neutral and weakly basic compounds. The …

Authors

Gupta RN; Hansebout RR

Journal

Journal of Chromatography B, Vol. 677, No. 1, pp. 183–189

Publisher

Elsevier

Publication Date

February 1996

DOI

10.1016/0378-4347(95)00426-2

ISSN

1570-0232