Journal article
Identification of endoplasmic reticulum in the primitive eukaryote Giardia lamblia using cryoelectron microscopy and antibody to BiP
Abstract
Giardia lamblia trophozoites contain a complex endomembrane system as demonstrated by fluorescence and cryoelectron microscopy. The endomembrane system was weakly detected in live cells using the fluorescent membrane dye 3,3'-dihexyloxacarbocyanine iodide. The definitive identification of endoplasmic reticulum required the development of a molecular label. We expressed Giardial Bip in Escherichia coli and raised a polyclonal antibody to the …
Authors
Soltys BJ; Falah M; Gupta RS
Journal
Journal of Cell Science, Vol. 109, No. 7, pp. 1909–1917
Publisher
The Company of Biologists
Publication Date
July 1, 1996
DOI
10.1242/jcs.109.7.1909
ISSN
0021-9533