Sodium Butyrate and a T Lymphocyte Cell Line-Derived Differentiation Factor Induce Basophilic Differentiation of the Human Promyelocy tic Leukemia Cell Line HL

Abstract Sodium butyrate induces basophilic differentiation of HL-60 promyelocytic leukemia cells that have been previously passaged in alkaline medium. A factor present in Mo conditioned medium (Mo-CM) acts synergistically with sodium butyrate to promote basophilic maturation in a dose-dependent fashion. The induced HL-60 cells exhibit nuclei at various stages of maturity and cytoplasmic granules staining azurophilic with …