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Antibodies to Indolealkylamines; Serotonin and...
Journal article

Antibodies to Indolealkylamines; Serotonin and Melatonin

Abstract

Serotonin, N-acetyl serotonin, and 5-methoxy-N-acetyl serotonin (melatonin) were conjugated to bovine serum albumin (BSA) using formaldehyde. The molar ratio of hapten to protein was determined spectrophotometrically. Spectrophotometric data indicated that serotonin and N-acetyl serotonin but not melatonin were conjugated to bovine serum albumin. Selected hapten–protein conjugates were suspended in Freund's adjuvant and injected into rabbits. …

Authors

Grota LJ; Brown GM

Journal

Biochemistry and Cell Biology, Vol. 52, No. 3, pp. 196–202

Publisher

Canadian Science Publishing

Publication Date

March 1, 1974

DOI

10.1139/o74-032

ISSN

0829-8211