Modulation of vascular function by perivascular adipose tissue: the role of endothelium and hydrogen peroxide
Journal Articles
Overview
Research
Identity
Additional Document Info
View All
Overview
abstract
Background and purpose:Perivascular adipose tissue (PVAT) attenuates vascular contraction, but the mechanisms remain largely unknown. The possible involvement of endothelium (E) and hydrogen peroxide (H2O2) was investigated.Experimental approach:Aortic rings from Wistar rats were prepared with both PVAT and E intact (PVAT+E+), with either PVAT or E removed (PVAT‐E+, or PVAT+E‐), or with both removed (PVAT‐E‐) for functional studies. Nitric oxide (NO) production was measured.Key results:Contraction to phenylephrine and 5‐HT respectively was highest in PVAT‐E‐, lowest in PVAT+E+, and intermediate in PVAT+E‐ or PVAT‐E+. In bioassay experiments, transferring bathing solution incubated with a PVAT+ ring (donor) to a PVAT‐ ring (recipient) induced relaxation in the recipient. This relaxation was abolished by E removal, NO synthase inhibition, scavenging of NO, high extracellular K+, or blockade of calcium‐dependent K+ channels (KCa). The solution stimulated NO production in isolated endothelial cells and in PVAT‐E+ rings. In E‐ rings, the contraction to phenylephrine of PVAT+ rings but not PVAT‐ rings was enhanced by catalase or soluble guanylyl cyclase (sGC) inhibitor, but reduced by superoxide dismutase and tiron. In PVAT‐E‐ rings, H2O2 attenuated phenylephrine‐induced contraction. This effect was counteracted by sGC inhibition. NO donor and H2O2 exhibited additive inhibition of the contraction to phenylephrine in PVAT‐E‐ rings.Conclusion:PVAT exerts its anti‐contractile effects through two distinct mechanisms: (1) by releasing a transferable relaxing factor which induces endothelium‐dependent relaxation through NO release and subsequent KCa channel activation, and (2) by an endothelium‐independent mechanism involving H2O2 and subsequent activation of sGC.British Journal of Pharmacology (2007) 151, 323–331; doi:10.1038/sj.bjp.0707228
