Use of Plasma Segments for Estimating Factor VIII Activity in Pools of Fresh Frozen Plasma

This study describes a procedure for estimating the factor VIII activity of pools of fresh frozen plasma destined for fractionation into factor VIII concentrates. This information is useful for estimating overall yields. We compared the factor VIII activity of plasma packs and the attached segments shortly after the production of fresh frozen plasmas, during storage at -30 degrees C for up to 4 weeks, after shipping at -20 degrees C to the fractionation site, after storage at -30 degrees C at the fractionation site, and after cryoprecipitation. The factor VIII activity of the segments and the plasma packs were indistinguishable at all stages except after cryoprecipitation. Our results suggest that, prior to the cryoprecipitation stage, a pool of a representative number of plasma segments can be used to determine the factor VIII activity of the plasma pool to be fractionated.