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Activation of factor V during intrinsic and...
Journal article

Activation of factor V during intrinsic and extrinsic coagulation. Inhibition by heparin, hirudin and d-Phe-Pro-Arg-Ch2Cl

Abstract

The validity of the hypothesis that Factor Xa activates Factor V in heparinized plasma was examined by establishing the temporal relationships between Factor V proteolysis and prothrombin consumption in plasma. Factor V was cleaved into Factor Va heavy chain (approx. 110 kDa) and an intermediate (approx. 230 kDa) 30 s after CaCl2 was added to contact-activated plasma (CAP). The larger fragment was converted into Factor V activation peptide …

Authors

Yang XJ; Blajchman MA; Craven S; Smith LM; Anvari N; Ofosu FA

Journal

Biochemical Journal, Vol. 272, No. 2, pp. 399–406

Publisher

Portland Press

Publication Date

December 1, 1990

DOI

10.1042/bj2720399

ISSN

0264-6021