Journal article
Entrapment of horseradish peroxidase in sugar-modified silica monoliths: Toward the development of a biocatalytic sensor
Abstract
A miniaturized HRP-entrapped bioreactor was prepared by a one-step enzyme immobilization method using a biocompatible sol-gel processing method employing either diglycerylsilane (DGS) or sodium silicate (SS) as precursors and a covalently tethered sugar, N-(3-triethoxysilylpropyl)gluconamide (GLS) as a silica modifier. Factors such as leaching, catalytic efficiency and long-term stability were examined to assess the role of the precursor and …
Authors
Lin T-Y; Wu C-H; Brennan JD
Journal
Biosensors and Bioelectronics, Vol. 22, No. 9-10, pp. 1861–1867
Publisher
Elsevier
Publication Date
4 2007
DOI
10.1016/j.bios.2006.04.026
ISSN
0956-5663