Catalysis and Rational Engineering of trans‐Acting pH6DZ1, an RNA‐Cleaving and Fluorescence‐Signaling Deoxyribozyme with a Four‐Way Junction Structure

Biosensor made of a catalytic DNA. When the RNA moiety (red) in the fluorogenic DNA substrate (purple) is cleaved by the bound catalytic DNA (blue) with a multiduplex structure, a strong fluorescence signal is generated owing to the separation of the fluorophore (F) from the quencher (Q).