Journal article
Efficacy of an Extraction Solvent Used to Quantify Albumin Deposition on Hydrogel Contact Lens Materials
Abstract
OBJECTIVES: Extracting proteins from conventional hydrogel (CH) and silicone hydrogel (SH) contact lens materials using a mixture of trifluoroacetic acid/acetonitrile (TFA/ACN) is a well-established procedure for quantifying individual and total protein deposited on contact lenses. The purpose of this study was to determine the efficacy of TFA/ACN in extracting albumin from SH and a CH group IV lens material using an in vitro model.
METHODS: …
Authors
Subbaraman LN; Glasier MA; Sheardown H; Jones L
Journal
Eye & Contact Lens Science & Clinical Practice, Vol. 35, No. 2, pp. 76–80
Publisher
Wolters Kluwer
Publication Date
March 2009
DOI
10.1097/icl.0b013e318199aff6
ISSN
1542-2321