Journal article
Cooperation in signal transduction of extracellular guanosine 5′ triphosphate and nerve growth factor in neuronal differentiation of PC12 cells
Abstract
Guanosine 5' triphosphate (GTP), acting synergistically with the nerve growth factor (NGF), enhances the proportion of neurite-bearing cells in cultures of PC12 rat pheochromocytoma cells. We studied the transduction mechanisms activated by GTP in PC12 cells and found that addition of GTP (100 microM) increased intracellular calcium concentration ([Ca(2+)](i)) in cells that were between 60 and 70% confluent. Addition of GTP also enhanced …
Authors
Guarnieri S; Fanò G; Rathbone MP; Mariggiò MA
Journal
Neuroscience, Vol. 128, No. 4, pp. 697–712
Publisher
Elsevier
Publication Date
January 2004
DOI
10.1016/j.neuroscience.2004.06.073
ISSN
0306-4522
Associated Experts
Fields of Research (FoR)
Medical Subject Headings (MeSH)
AnimalsBarbituratesBlotting, WesternCalciumCalcium Channel BlockersCell CountCell DifferentiationChelating AgentsClotrimazoleDiagnostic ImagingDose-Response Relationship, DrugDrug SynergismEgtazic AcidEnzyme InhibitorsExtracellular SpaceFluorescent Antibody TechniqueFluorescent DyesGallic AcidGrowth InhibitorsGuanosine TriphosphateIonomycinIonophoresIsoxazolesMembrane PotentialsMicroscopy, ConfocalMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Nerve Growth FactorNeuritesNifedipinePC12 CellsPertussis ToxinPyridoxal PhosphateRatsSignal TransductionSuraminTime FactorsTriazines