Journal article
The acute-phase response of cultured rat hepatocytes. System characterization and the effect of human cytokines
Abstract
Hepatocytes were isolated from adult livers and cultured for periods of up to 5 days as monolayers at an initial density of 10(6) cells/10cm2 in Williams E medium containing insulin, dexamethasone and 5% foetal-calf serum. The daily production of 11 plasma proteins was measured by electroimmunoassay and compared with the concentrations of the same proteins in the plasma of normal rats and of those with experimental inflammation. Hepatocytes …
Authors
Koj A; Gauldie J; Regoeczi E; Sauder DN; Sweeney GD
Journal
Biochemical Journal, Vol. 224, No. 2, pp. 505–514
Publisher
Portland Press
Publication Date
December 1, 1984
DOI
10.1042/bj2240505
ISSN
0264-6021