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Localization of membrane proteins by the use of a...
Journal article

Localization of membrane proteins by the use of a photoreactive fatty acid incorporated in vivo into vesicular stomatitis virus.

Abstract

Vesicular stomatitis virus grown in the presence of omega-[9-3H]diazirinophenoxy nonanoate resulted in the biosynthetic incorporation of this photoreactive fatty acid into viral phospholipids as well as into the membrane anchoring domain of the viral G glycoprotein. Photolysis of the isolated virus at 360 nm resulted in extensive labeling of the G protein but none of the other proteins by the viral phospholipids. In addition, a new product was …

Authors

Capone J; Leblanc P; Gerber GE; Ghosh HP

Journal

Journal of Biological Chemistry, Vol. 258, No. 3, pp. 1395–1398

Publisher

Elsevier

Publication Date

February 1983

DOI

10.1016/s0021-9258(18)32994-6

ISSN

0021-9258