Journal article
Tritiation of commercial heparins by reaction with NaB3H4: Chemical analysis and biological properties of the product
Abstract
A simple method to tritiate commercial samples of heparin is described. Heparin is reacted with NaB3H4 at pH 8.0 for 3 h at room temperature, after which the 3H-labeled mucopolysaccharide is isolated by gel filtration. Using NaB3H4 of low specific radioactivity (227–555 mCi/mmol), [3H]heparins containing 3.7–8.0 × 105 dpm/mg are made whereas with NaB3H4 of higher specific activity (5–10 Ci/mmol), preparations of 1.2–1.8 × 107 dpm/mg are …
Authors
Hatton MWC; Berry LR; Machovich R; Regoeczi E
Journal
Analytical Biochemistry, Vol. 106, No. 2, pp. 417–426
Publisher
Elsevier
Publication Date
8 1980
DOI
10.1016/0003-2697(80)90542-4
ISSN
0003-2697