Journal article
Multiplex PCR for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in Genitourinary specimens
Abstract
We developed a multiplex PCR (M-PCR) assay for the simultaneous detection of Chlamydia trachomatis and Neisseria gonorrhoeae. M-PCR employed C. trachomatis-specific primers KL1-KL2 and N. gonorrhoeae-specific primers HO1-HO3 and produced products of 241 and 390 bp, respectively. PCR products were easily detected by agarose gel electrophoresis and confirmed by Southern hybridization using labelled oligonucleotide probes. M-PCR had a sensitivity …
Authors
Mahony JB; Luinstra KE; Tyndall M; Sellors JW; Krepel J; Chernesky M
Journal
Journal of Clinical Microbiology, Vol. 33, No. 11, pp. 3049–3053
Publisher
American Society for Microbiology
Publication Date
November 1995
DOI
10.1128/jcm.33.11.3049-3053.1995
ISSN
0095-1137
Associated Experts
Fields of Research (FoR)
Medical Subject Headings (MeSH)
AdolescentAdultBlotting, SouthernCervix UteriChlamydia InfectionsChlamydia trachomatisDiagnosis, DifferentialFemaleFemale Urogenital DiseasesGonorrheaHumansKenyaMaleMale Urogenital DiseasesOligonucleotide ProbesOntarioPolymerase Chain ReactionReproducibility of ResultsSensitivity and SpecificityUrethraUrine