Generation of Murine Xenograft Models of Brain Tumors from Primary Human Tissue for In Vivo Analysis of the Brain Tumor-Initiating Cell Academic Article uri icon

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abstract

  • The generation of xenograft models, which support the growth of human tissue in animals, forms an important part of a researcher's tool kit and enhances the ability to understand the initiation and development of cancer in vivo. Especially in the context of the brain tumor-initiating cell (BTIC), a xenograft model allows for careful characterization of BTIC roles in tumor initiation, growth, and relapse. Here, we detail a set of procedures which describe the isolation, enrichment, and intracranial injection of human BTICs from patient samples to generate xenograft models of a human brain tumor.

authors

  • Qazi, Maleeha
  • Mann, Aneet
  • van Ommeren, Randy
  • Venugopal, Chitra
  • McFarlane, Nicole
  • Vora, Parvez
  • Singh, Sheila

publication date

  • 2014