Development of a Patient-Derived Xenograft Model Using Brain Tumor Stem Cell Systems to Study Cancer Journal Articles

abstract

• Patient-derived xenograft (PDX) models provide an excellent platform to understand cancer initiation and development in vivo. In the context of brain tumor initiating cells (BTICs), PDX models allow for characterization of tumor formation, growth, and recurrence, in a clinically relevant in vivo system. Here, we detail procedures to harvest, culture, characterize, and orthotopically inject human BTICs derived from patient samples.

authors

• Chokshi, C
• Dhillon, M
• McFarlane, N
• Venugopal, C
• Singh, Sheila

status

• published 

publication date

• 2016

has subject area

• 0399 Other Chemical Sciences (FoR)
• 0601 Biochemistry and Cell Biology (FoR)
• Animals (MeSH)
• Biomarkers (MeSH)
• Brain Neoplasms (MeSH)
• Cell Self Renewal (MeSH)
• Developmental Biology (Science Metrix)
• Disease Models, Animal (MeSH)
• Flow Cytometry (MeSH)
• Heterografts (MeSH)
• Humans (MeSH)
• Immunohistochemistry (MeSH)
• Mice (MeSH)
• Neoplastic Stem Cells (MeSH)
• Spheroids, Cellular (MeSH)
• Tumor Cells, Cultured (MeSH)
• Tumor Stem Cell Assay (MeSH)

published in

• Methods in molecular biology (Clifton, N.J.)  Journal

edition

• 1458

keywords

• Animals
• Biomarkers
• Brain Neoplasms
• Brain tumor-initiating cell (BTIC)
• Cell Self Renewal
• Disease Models, Animal
• Flow Cytometry
• Flow cytometry
• Heterografts
• Humans
• Immunohistochemistry
• In vitro proliferation assay
• In vitro self-renewal assay
• Intracranial injections
• Lentivirus
• Mice
• NOD-SCID
• Neoplastic Stem Cells
• Patient-derived xenograft (PDX)
• Spheroids, Cellular
• Tumor Cells, Cultured
• Tumor Stem Cell Assay

Digital Object Identifier (DOI)

• 10.1007/978-1-4939-3801-8_17

PubMed ID

• 27581026

start page

• 231

end page

• 245

volume

• 1458