Journal article
Endonuclease Activities Associated with High Molecular Weight and Internucleosomal DNA Fragmentation in Apoptosis
Abstract
We have used pulsed-field gel electrophoresis and electron microscopy to correlate stages of DNA fragmentation with alterations of nuclear structure during apoptosis. DNA fragmentation occurs in two stages. The first is initiated by a previously undescribed endonucleolytic activity that cleaves DNA into 50- to 300-kb fragments. Electron microscopy showed that this degree of cleavage was sufficient to cause the chromatin to undergo condensation. …
Authors
Walker PR; Weaver VM; Lach B; LeBlanc J; Sikorska M
Journal
Experimental Cell Research, Vol. 213, No. 1, pp. 100–106
Publisher
Elsevier
Publication Date
July 1994
DOI
10.1006/excr.1994.1178
ISSN
0014-4827