Complete sequences of a novel blaNDM-1-harbouring plasmid from Providencia rettgeri and an FII-type plasmid from Klebsiella pneumoniae identified in Canada Academic Article uri icon

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abstract

  • OBJECTIVES: Emergence of plasmids harbouring bla(NDM-1) is a major public health concern due to their association with multidrug resistance and their potential mobility. METHODS: PCR was used to detect bla(NDM-1) from clinical isolates of Providencia rettgeri (PR) and Klebsiella pneumoniae (KP). Antimicrobial susceptibilities were determined using Vitek 2. The complete DNA sequence of two bla(NDM-1) plasmids (pPrY2001 and pKp11-42) was obtained using a 454-Genome Sequencer FLX. Contig assembly and gap closures were confirmed by PCR-based sequencing. Comparative analysis was done using BLASTn and BLASTp algorithms. RESULTS: Both clinical isolates were resistant to all β-lactams, carbapenems, aminoglycosides, ciprofloxacin and trimethoprim/sulfamethoxazole, and susceptible to tigecycline. Plasmid pPrY2001 (113 295 bp) was isolated from PR. It did not show significant homology to any known plasmid backbone and contained a truncated repA and novel repB. Two bla(NDM-1)-harbouring plasmids from Acinetobacter lwoffii (JQ001791 and JQ060896) shared 100% similarity to a 15 kb region that contained bla(NDM-1). pPrY2001 also contained a type II toxin/antitoxin system. pKp11-42 (146 695 bp) was isolated from KP. It contained multiple repA genes. The plasmid backbone had the highest homology to the IncFIIk plasmid type (51% coverage, 100% nucleotide identity). The bla(NDM-1) region was unique in that it was flanked upstream by IS3000 and downstream by a novel transposon designated Tn6229. pKp11-42 also contained a number of mutagenesis and plasmid stability proteins. CONCLUSIONS: pPrY2001 differed from all known plasmids due to its novel backbone and repB. pKp11-42 was similar to IncFIIk plasmids and contained a number of genes that aid in plasmid persistence.

authors

  • Mataseje, LF
  • Boyd, DA
  • Lefebvre, B
  • Bryce, E
  • Embree, J
  • Gravel, D
  • Katz, K
  • Kibsey, P
  • Kuhn, M
  • Langley, J
  • Mitchell, R
  • Roscoe, D
  • Simor, A
  • Taylor, G
  • Thomas, E
  • Turgeon, N
  • Mulvey, MR
  • Boyd, D
  • Bryce, E
  • Conly, J
  • Deheer, J
  • Embil, J
  • Embree, J
  • Evans, G
  • Forgie, S
  • Frenette, C
  • Lemieux, C
  • Golding, G
  • Gravel, D
  • Henderson, E
  • Hutchinson, J
  • John, M
  • Johnston, L
  • Katz, K
  • Kibsey, P
  • Kuhn, M
  • Langley, J
  • Lesaux, N
  • Loeb, Mark
  • Matlow, A
  • McGeer, A
  • Miller, M
  • Mitchell, R
  • Moore, D
  • Mounchili, A
  • Mulvey, M
  • Pelude, L
  • Roth, V
  • Simor, A
  • Suh, K
  • Taylor, G
  • Thomas, E
  • Turgeon, N
  • Vearncombe, M
  • Vayalumkal, J
  • Weiss, K
  • Wong, A

publication date

  • March 1, 2014