Regional localization of 18 human X-linked DNA sequences Academic Article uri icon

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abstract

  • A series of human probes with unique sequences has been isolated from a recombinant phage library constructed with DNA obtained from a human-hamster hybrid cell line. This cell line contained the X chromosome as the only human component. For 18 of these probes, a human X-chromosome origin has been confirmed and they have been regionally assigned by a combination of techniques: dosage studies utilizing DNA from human fibroblasts carrying X-chromosome duplications and deletions; the presence or absence of hybridization to digested DNA from hybrid lines carrying fragments of the X chromosome; and in situ hybridization to metaphase chromosomes. The use of dosage as a means to regionally assign probes significantly improves resolution of the X chromosome.

authors

  • Riddell, DC
  • Wang, HS
  • Beckett, J
  • Chan, A
  • Holden, JJA
  • Mulligan, LM
  • Phillips, MA
  • Simpson, NE
  • Wrogemann, K
  • Hamerton, JL
  • White, Bradley

publication date

  • 1986