Conjugation of Enzymes to Antibodies Journal Articles

abstract

• AbstractConjugation of enzymes to antibodies involves the formation of a stable, covalent linkage between an enzyme [e.g., horseradish peroxidase (HRPO), urease, or alkaline phosphatase] and an antigen‐specific monoclonal or polyclonal antibody in which neither the antigen‐combining site of the antibody nor the active site of the enzyme is functionally altered. This unit describes procedures for cross‐linking HRPO, urease or alkaline phosphatase to immunoaffinity‐purified monoclonal or polyclonal antibodies (IgG).

authors

• Winston, Scott E
• Fuller, Steven A
• Evelegh, Michael
• Hurrell, John GR

status

• published 

publication date

• April 2000

has subject area

• Antibodies (MeSH)
• Biochemistry & Molecular Biology (Science Metrix)
• Enzymes (MeSH)
• Immunoenzyme Techniques (MeSH)
• Staining and Labeling (MeSH)

published in

• Current Protocols in Molecular Biology  Journal

keywords

• Antibodies
• Enzymes
• Immunoenzyme Techniques
• Staining and Labeling

Digital Object Identifier (DOI)

• 10.1002/0471142727.mb1101s50

PubMed ID

• 18265067

start page

• Unit11.1

volume

• 50

issue

• 1