Journal article
Application of image analysis on monolayer cultures of rat hepatocytes: assessment of a chemically inducible G0-G1 cell cycle phase shift.
Abstract
The phenobarbital induced shift from G0 to G1 cell cycle phases was analyzed in freshly isolated cultured rat hepatocytes by image analysis. Nuclei in situ in monolayers or in an isolated state were stained with quinacrine dihydrochloride. Fluorescence intensity and fluorescence area were recorded in controls and after treatment with phenobarbital (1.5 or 3 mM, 48 h). Reproducible measurements were obtained with the aid of an elaborate …
Authors
Holderegger C; Duivenvoorden WC; Maier P
Journal
Analytical Cellular Pathology, Vol. 4, No. 2, pp. 87–101
Publication Date
March 1992
ISSN
2210-7177