Lipoprotein enrichment in orange insoluble particulate matter reproducibly appearing in cryoprecipitate

Background and Objective Cryoprecipitate prepared from two whole blood donations from the same donor contained insoluble orange particulate material (OPM). We sought to identify the OPM.Materials and Methods OPM was recovered from the blood product by centrifugation, dissolved in sodium dodecyl sulphate (SDS) and analysed by SDS–polyacrylamide gel electrophoresis and immunoblotting.Results Solubilized OPM was enriched in apolipoproteins B and E, but not apolipoprotein A1, immunoglobulin G or albumin, suggesting lipoprotein enrichment in OPM. Subsequent clinical laboratory blood tests confirmed low‐density lipoprotein hyperlipidaemia with normal triglyceride levels. Further, cryoprecipitate production from this donor was prevented by implementation of national predominantly male plasma policies.Conclusion Cryoprecipitate produced from hyperlipidaemic donors may contain insoluble particles that render it inappropriate for transfusion.

Lipoprotein enrichment in orange insoluble particulate matter reproducibly appearing in cryoprecipitate Journal Articles