A Carrier Phase Ultrafiltration and Backflow Recovery Technique for Purification of Biological Macromolecules

A simple carrier phase based ultrafiltration technique that is akin to liquid chromatography and is suitable for medium-to-large volume sample preparation in the laboratory is discussed in this paper. A membrane module was integrated with a liquid chromatography system in a "plug and play" mode for ease of sample handling, and recovery of species retained by the membrane. The sample injector and pump were used for feed injection and for driving …