Drosophila melanogaster wing images from genetic manipulations of planar cell polarity and tissue growth genes (UAS-genex.RNAi and NP6333-GAL4) Datasets uri icon

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abstract

  • These are images of Drosophila melanogaster wings from RNAi knockdowns (using VDRC RNAi lines) crossed to the NP6333-GAL4 line (also known as the Pen-GAL4 line). These crosses (and images) were done just to test the lines. The UAS-narrow.RNAi did not seem to work, but all the other ones did.Crosses were performed by Runyang Yin in the Dworkin lab at McMaster University.All crosses were done at 24°C in a Percival Growth Chamber (12:12 light cycle). Adult flies were stored in 70% ethanol prior to dissection in 70% glycerol/PBS (with a drop of phenol as a bacterio-static agent).All images were taken on an Olympus BX-43 microscope with a 4X objective (40X total magnification). The objective is UPlanSApo (NA 0.16. FN26.5). Images were captured on an Olympus DP80 digital camera using Olympus cellSenStandard software (v1.14). Image resolution pixels: 4080 x 3072Image size actual: 2.2 mm x 1.7 mm537.5 nm/pixel24bit RGB (8 bit/channel)genes manipulatedfatnarrow (not really PCP or growth, but has an effect on morphogenesis). Note this construct did not seem to do much, so these pictures may not really be a manipulation of narrow. We re-ordered it and the new strain had much clearer effects.starry night (stan)Van Gogh (vang)