Journal article
A Rapid Sputum‐based Lateral Flow Assay for Airway Eosinophilia using an RNA‐cleaving DNAzyme Selected for Eosinophil Peroxidase
Abstract
The first protein-binding allosteric RNA-cleaving DNAzyme (RCD) obtained by direct in vitro selection against eosinophil peroxidase (EPX), a validated marker for airway eosinophilia, is described. The RCD has nanomolar affinity for EPX, shows high selectivity against related peroxidases and other eosinophil proteins, and is resistant to degradation by mammalian nucleases. An optimized RCD was used to develop both fluorescence and lateral flow …
Authors
Ali MM; Mukherjee M; Radford K; Patel Z; Capretta A; Nair P; Brennan JD
Journal
Angewandte Chemie International Edition, Vol. 62, No. 38,
Publisher
Wiley
Publication Date
September 18, 2023
DOI
10.1002/anie.202307451
ISSN
1433-7851