Exploring Proton-Only NMR Experiments and Filters for Daphnia In Vivo: Potential and Limitations

Environmental metabolomics provides insight into how anthropogenic activities have an impact on the health of an organism at the molecular level. Within this field, in vivo NMR stands out as a powerful tool for monitoring real-time changes in an organism's metabolome. Typically, these studies use 2D ¹³C-¹H experiments on ¹³C-enriched organisms. Daphnia are the most studied species, given their widespread use in toxicity testing. However, with …