CE‐MS for Clinical Proteomics and Metabolomics: Strategies and Applications

This chapter highlights the potential of capillary electrophoresis‐mass spectrometry (CE‐MS) for clinical proteomics and metabolomics studies. CE‐MS has been used for the characterization of a wide range of analytes, including (endogenous) metabolites, drug‐related compounds and proteins, as well as intact cells and subcellular components (i.e., organelles). The chapter devotes to the use of recently developed CE‐MS methodologies relevant to clinical applications as related to proteomics and metabolomics studies. Concerning proteomics, the focus will be on bottom‐up proteomics studies (i.e., the analysis of (complex) peptide mixtures obtained after enzymatic digestion of the protein sample) and the profiling of native peptides in biological samples. In general, the CE‐MS approaches reported in the chapter offers a promising approach for clinical proteomics and metabolomics notably when used in conjunction with accelerated data workflows for biomarker discovery based on multiplexed separations.