Journal article
Properties of tRNAPhe from Drosophila
Abstract
1. Drosophila tRNAPhe is not retarded on BD-cellulose columns as are other cytoplasmic tRNAPhe's from eukaryotes.2. Neither purified tRNAPhe nor crude tRNA has the characteristic fluorescence of tRNAs containing Y-type bases. A nucleotide analysis of purified tRNAPhe detected no Y-type base and in this respect it is similar to prokaryotic and organelle tRNAPhe's.3. Aminoacylations of Drosophila tRNA with [14C]phenylalanine using heterologous …
Authors
White BN; Tener GM
Journal
Biochimica et Biophysica Acta, Vol. 312, No. 2, pp. 267–275
Publisher
Elsevier
Publication Date
6 1973
DOI
10.1016/0005-2787(73)90372-9
ISSN
0006-3002
Associated Experts
Fields of Research (FoR)
Medical Subject Headings (MeSH)
Amino Acyl-tRNA SynthetasesAnimalsBase SequenceBenzoatesCarbon IsotopesChromatography, DEAE-CelluloseChromatography, Thin LayerDrosophila melanogasterEscherichia coliFluorescencePhenylalanineRNA, TransferRibonucleasesRibonucleotidesSaccharomyces cerevisiaeSpecies SpecificitySpectrophotometry, UltravioletTransfer RNA Aminoacylation