Development of a Pseudomonas syringae–Eutrema salsugineum pathosystem to investigate disease resistance in a stress tolerant extremophile model plant Journal Articles uri icon

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abstract

  • To improve the ability to understand how plants respond to multiple and/or concurrent stresses, disease resistance was investigated in Eutrema salsugineum, an extremophile model plant that is highly tolerant of abiotic stress. Compared to Arabidopsis (Col‐0), both Yukon and Shandong Eutrema accessions exhibit increased resistance to Pseudomonas syringae pv. tomato DC3000 (Pst) and pv. maculicola (Psm), with Shandong Eutrema exhibiting greater resistance to Pst than Yukon Eutrema. RT‐PCR of the EsPR1 (Pathogenesis‐related 1) defence marker gene confirmed RNA‐Seq data that healthy Shandong Eutrema constitutively expresses EsPR1. The data suggests that Shandong Eutrema exists in a highly primed state of defence preparedness, as it displays heightened resistance compared to defence‐primed natural accessions of Arabidopsis (Can‐0, Bur‐0). Pathogen‐triggered PR1 expression was delayed in Yukon Eutrema; however, these plants were resistant to Pst suggesting that Yukon Eutrema employs a PR1‐independent mechanism to resist Pst. This study demonstrates that Eutrema is an excellent model to investigate biotic stress tolerance. The Eutrema–P. syringae pathosystem will facilitate future studies to understand how this extremophile tolerates both abiotic and biotic stress, and will allow exploration of the interplay of these responses to inform efforts to improve stress tolerance in crops.

publication date

  • April 2015